Structure of Membrane-Embedded M13 Major Coat Protein Is Insensitive to Hydrophobic Stress
نویسندگان
چکیده
منابع مشابه
Quantification of Protein-Lipid Selectivity using FRET: Application to the M13 Major Coat Protein.
Quantification of lipid selectivity by membrane proteins has been previously addressed mainly from electron spin resonance studies. We present here a new methodology for quantification of protein-lipid selectivity based on fluorescence resonance energy transfer. A mutant of M13 major coat protein was labeled with 7-diethylamino-3((4'iodoacetyl)amino)phenyl-4-methylcoumarin to be used as the don...
متن کاملMutational analysis of the major coat protein of M13 identifies residues that control protein display.
We have reported variants of the M13 bacteriophage major coat protein (P8) that enable high copy display of monomeric and oligomeric proteins, such as human growth hormone and steptavidin, on the surface of phage particles (Sidhu SS, Weiss GA, Wells JA. 2000. High copy display of large proteins on phage for functional selections. J Mol Biol 296:487-495). Here, we explore how an optimized P8 var...
متن کاملStructural and orientational information of the membrane embedded M13 coat protein by (13)C-MAS NMR spectroscopy.
Oriented and unoriented M13 coat protein, incorporated into dimyristoyl phosphatidylcholine bilayers, has been studied by (13)C-magic angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy. Rotational resonance experiments provided two distance constraints between Calpha and C&z.dbnd6;O positions of the labelled residues Val-29/Val-30 (0.4+/-0.5nm) and Val-29/Val-31 (0.45+/-0. 5nm) in...
متن کاملConstrained modeling of spin-labeled major coat protein mutants from M13 bacteriophage in a phospholipid bilayer.
The family of three-dimensional molecular structures of the major coat protein from the M13 bacteriophage, which was determined in detergent micelles by NMR methods, has been analyzed by constrained geometry optimization in a phospholipid environment. A single-layer solvation shell of dioleoyl phosphatidylcholine lipids was built around the protein, after replacing single residues by cysteines ...
متن کاملThe Membrane-Bound Form of Gene 9 Minor Coat Protein of Bacteriophage M13
eindelijk die kast opruimen!). Summary 108 Samenvatting 111 Curriculum vitae 115 List of Publications 117 Het in dit proefschrift beschreven onderzoek werd mogelijk gemaakt met financiële steun van de Stichting Levenswetenschappen (SLW) van de Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO). Abbreviations ATR-FTIR attenuated total reflection Fourier transform infrared CD circular ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2007
ISSN: 0006-3495
DOI: 10.1529/biophysj.107.112698